Title: Perform polymerase chain reaction (PCR) testing.
Course: Medical Laboratory Technology II (14102-II) > Section: Understanding the Basics of Molecular Diagnostics

Performance should include

- explaining that PCR testing is a relatively quick procedure for analyzing DNA without the need for cloning and that it is commonly used for diagnosing genetic diseases
- following the steps for PCR testing

  - reagent preparation
  - equipment preparation
  - proper loading of the gel
  - proper use of the PCR thermocycler.

Process/Skill Questions:

- What is a nucleotide? What is the role of nucleotides in PCR testing?
- Why are amino acid sequences important in PCR?
- How can a DNA segment be replicated?
- What was the purpose of the Human Genome Project?
- At what temperature does denaturization occur?
- What buffer is used in PCR testing?
- What is the principle of a thermocycler?