Title: Investigate an agricultural biotechnology lab process.
Course: Biotechnology Applications in Agriculture (18308-III) > Section: Applying Biotechnology to Agricultural Species

Investigation may include

- DNA extraction
- polymerase chain reaction (PCR)
- gel electrophoresis
- spectrophotometry
- creation of transgenic organisms
- gene knockout technology
- clustered regularly interspaced short palindromic repeats (CRISPR) system for gene editing
- transgene-free gene editing
- use of somatic cell nuclear transfer (i.e., cloning).

Process/Skill Questions:

- What steps are taken to plan and develop a plant field trial?
- Why is it important to conduct and collect data from field trials?
- What can be learned from analyzing data collected from a field trial?
- What is the difference between cloning from embryos and cloning from differentiated cells?
- What are the steps that ensure safety throughout this process?